Journal: Cardiology Research and Practice

Article Title: Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients

doi: 10.1155/2019/8271871

Figure Lengend Snippet: Comparison in serum anti-M2-R levels among three groups. The serum anti-M2-R level in the AF group was 496.2 ± 232.5 pmol/L, which was significantly higher than 86.3 ± 25.7 pmol/L in the non-AF group, p < 0.001. No difference in serum anti-M2-R levels between the non-AF and control group was observed (86.3 ± 25.7 vs. 82.4 ± 34.9 pmol/L, p =0.654).

Article Snippet: M2 receptor antibody (ab2805, Abcam, Cambridge, USA), TGF- β 1 antibody (ab92486, Abcam), and CTGF antibody (ab6992, Abcam) were added at dilutions of 1 : 100, 1 : 1,000, and 1 : 400, respectively, followed by incubation for 60 min at room temperature and washing three times with phosphate-buffered saline (PBS) solution.

Techniques:

Journal: Cardiology Research and Practice

Article Title: Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients

doi: 10.1155/2019/8271871

Figure Lengend Snippet: Immunohistochemical analysis of M2 receptor, TGF- β 1, and CTGF expression. Representative sections of the immunohistochemistry- (IHC-) stained left atrial appendage tissue of patients in the non-AF and AF group. (a) M2 receptor; (b) TGF- β 1; (c) CTGF.

Article Snippet: M2 receptor antibody (ab2805, Abcam, Cambridge, USA), TGF- β 1 antibody (ab92486, Abcam), and CTGF antibody (ab6992, Abcam) were added at dilutions of 1 : 100, 1 : 1,000, and 1 : 400, respectively, followed by incubation for 60 min at room temperature and washing three times with phosphate-buffered saline (PBS) solution.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining

Journal: Cardiology Research and Practice

Article Title: Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients

doi: 10.1155/2019/8271871

Figure Lengend Snippet: Correlation between serum anti-M2-R levels and fibrosis-related indexes. Pearson correlation analysis showed that M2 receptor, CVF, and collagen I and collagen III were all correlated with serum anti-M2-R levels (M2 receptor: r = 0.90, p < 0.001; CVF: r = 0.84, p < 0.001; collagen I: r = 0.87, p < 0.001; collagen III: r = 0.78, p < 0.001). Fibrogenic indexes were also correlated with serum anti-M2-R levels (TGF- β 1: r = 0.92, p < 0.001; CTGF: r = 0.89, p < 0.001).

Article Snippet: M2 receptor antibody (ab2805, Abcam, Cambridge, USA), TGF- β 1 antibody (ab92486, Abcam), and CTGF antibody (ab6992, Abcam) were added at dilutions of 1 : 100, 1 : 1,000, and 1 : 400, respectively, followed by incubation for 60 min at room temperature and washing three times with phosphate-buffered saline (PBS) solution.

Techniques:

Journal: Oncotarget

Article Title: Deletion or inhibition of soluble epoxide hydrolase protects against brain damage and reduces microglia-mediated neuroinflammation in traumatic brain injury

doi: 10.18632/oncotarget.21139

Figure Lengend Snippet: (A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or arginase 1 (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).

Article Snippet: To assess proinflammatory (M1) and anti-inflammatory (M2) microglia/macrophages, sections were incubated overnight at 4°C with rabbit anti-Iba1 (1:1,000; Wako), together with rat anti-CD16/32 (a classical M1 activation marker; 1:100; BD Biosciences), mouse anti-CD206 (an alternative M2 activation marker; 1:100; Bio-Rad Laboratories, Hercules, CA, USA) or rabbit anti-arginase 1 (an alternative M2 activation marker; 1:100; BD Biosciences).

Techniques: Immunofluorescence, Activation Assay, Marker, Enzyme-linked Immunosorbent Assay